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The Extracellular Matrix01:42

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Multi-Scale Modification of Metallic Implants With Pore Gradients, Polyelectrolytes and Their Indirect Monitoring In vivo
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Customizable Implant-specific and Tissue-Specific Extracellular Matrix Protein Coatings Fabricated Using Atmospheric

Fei Tan1,2,3, Mohamed Al-Rubeai4

  • 1Department of Otolaryngology - Head & Neck Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

Frontiers in Bioengineering and Biotechnology
|October 23, 2019
PubMed
Summary

Atmospheric plasma coating of surgical implants with extracellular matrix proteins enhances biological functions. This novel surface biotechnology improves cell interactions and offers a versatile platform for future implant therapies.

Keywords:
atmospheric plasmabonecoatingcollagenimplantlamininnerve

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Area of Science:

  • Biomaterials Science
  • Surface Biotechnology
  • Regenerative Medicine

Background:

  • Surgical implant efficacy is limited by biological integration.
  • Surface modification is crucial for improving implant performance.
  • Extracellular matrix proteins hold potential for enhancing cellular responses.

Purpose of the Study:

  • To develop and characterize atmospheric plasma-generated extracellular matrix protein coatings on surgical implants.
  • To evaluate the impact of these coatings on cellular behavior and molecular profiles.
  • To establish atmospheric plasma as a versatile tool for implant surface functionalization.

Main Methods:

  • Atmospheric plasma coating with collagen and laminin.
  • X-ray Photoelectron Spectroscopy (XPS) and Scanning Electron Microscopy (SEM) for chemical and morphological analysis.
  • Contact angle goniometry and simulated body fluid (SBF) dissolution studies for material properties.
  • Cell adhesion, proliferation, and differentiation assays (enzymatic detachment, flow cytometry, ELISA) with human mesenchymal and neural stem cells (NSCs).
  • Pathway-specific PCR array analysis for gene expression profiling during osteogenesis and neurogenesis.

Main Results:

  • Robust collagen and laminin coatings were successfully fabricated using atmospheric plasma.
  • Coatings exhibited enhanced hydrophilicity and physiological durability in SBF.
  • Significant improvements in human mesenchymal and neural stem cell adhesion, proliferation, and differentiation were observed.
  • Dozens of genes related to osteogenesis and neurogenesis were found to be deregulated on the ECM-coated implants.
  • Atmospheric plasma enables customizable, tissue-specific implant coatings.

Conclusions:

  • Atmospheric plasma is a versatile technology for creating functionalized implant surfaces.
  • ECM protein coatings significantly enhance biological interactions and cellular functions.
  • The observed molecular profile changes provide insights for designing advanced implant-based therapies.