Nocardioides jejuensis sp. nov., isolated from soil

A Gram-stain positive, asporogenous, aerobic, white -coloured bacterium, designated 18JY15-6^T, was isolated from soil from Jeju Island, Korea. Pairwise analysis of the 16S rRNA gene sequence of strain 18JY15-6^T indicated high similarity to Nocardioides phosphati DSM 104026^T (97.4%), Marmoricola terrae KACC 17308^T (96.7%) and Nocardioides jensenii KCTC 0074BP^T (96.6%). Phylogenetic analysis revealed that strain 18JY15-6^T formed a distinct lineage within the family Nocardioidaceae and is closely related to members of the genus Nocardioides. Genome sequencing of strain 18JY15-6^T revealed 3221 total genes, including 3162 protein coding genes, 59 RNA and 31 pseudogenes. Growth was observed at 18-37 °C (optimal 30 °C) in R2A medium at pH 7.0. The major cellular fatty acids of strain 18JY15-6^T were identified as C_16:0, C_18:1ω9c, C_18:0 10-methyl, tuberculostearic and C_17:0. The fatty acid profile of strain 18JY15-6^T was more dissimilar when compared with M. terrae. The only respiratory quinone present was found to be MK-8(H₄). The major polar lipids are diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. The results of phylogenetic, biochemical and chemotaxonomic characterisation allow the differentiation of strain 18JY15-6^T from N. phosphati WYH11-7^T, M. terrae JOS5-1^T and N. jensenii NBRC 14755^T which supports the conclusion that this strain represents a novel species of the genus Nocardioides, for which we propose the name Nocardioides jejuensis sp. nov. The type strain of Nocardioides jejuensis is 18JY15-6^T (= KCTC 49105^T = JCM 33182^T).