Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Turkey acrosin. I. Isolation, purification, and partial characterization.

M E Richardson1, A B Bodine, D P Froman

  • 1Department of Poultry Science, College of Agricultural Sciences, Clemson University, South Carolina 29634.

Biology of Reproduction
|April 1, 1988
PubMed
Summary

Turkey spermatozoa acrosin, a serine protease glycoprotein, was purified and characterized. Researchers identified two isozymes and three subunits, advancing reproductive biology research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Association of gene variant type and location with breast cancer risk in the general population.

Annals of oncology : official journal of the European Society for Medical Oncology·2025
Same author

Effects of Package Temperature and Days of Storage on the Flavor Score of Processed Milk <sup>1</sup>.

Journal of food protection·2019
Same author

Deduction of a calcium ion circuit affecting rooster sperm in vitro.

Journal of animal science·2016
Same author

Evaluation of a semen extender from a bioenergetic perspective.

Journal of animal science·2015
Same author

Premises for fowl sperm preservation based on applied bioenergetics.

Journal of animal science·2014
Same author

Cytoplasmic droplets of painted turtle spermatozoa.

Journal of morphology·2013

Area of Science:

  • Reproductive Biology
  • Enzymology
  • Biochemistry

Background:

  • Acrosin is a key enzyme in sperm function, essential for fertilization.
  • Understanding avian acrosin aids comparative studies with mammalian species.

Purpose of the Study:

  • To extract and purify turkey acrosin.
  • To characterize its biochemical properties and subunit composition.

Main Methods:

  • Urea extraction, sonication, and freeze-thaw cycles for acrosin isolation.
  • Chromatofocusing and affinity chromatography for purification.
  • Polyacrylamide gel electrophoresis (PAGE) for isozyme and subunit analysis.

Main Results:

  • Acrosin was purified approximately 18-fold.

Related Experiment Videos

  • Turkey acrosin is a glycoprotein with serine protease characteristics.
  • Two isozymes were detected by PAGE, and SDS-PAGE revealed three subunits (11.7, 13.9, and 15.9 kDa).

    • Conclusions:

    • Chromatofocusing is an effective method for turkey acrosin purification.
    • Turkey acrosin shares properties with mammalian acrosins.
    • The identified subunits provide insights into the enzyme's structure.