Numerical investigation of adhesion dynamics of a deformable cell pair on an adhesive substrate in shear flow

Adhesion dynamics of cells is of great value to biological systems and adhesion-based biomedical applications. Although adhesion of a single cell or capsule has been widely studied, physical insights into the adhesion dynamics of aggregates containing two or more cells remain elusive. In this paper, we numerically investigate the dynamic adhesion of a deformable cell pair to a flat substrate under shear flow. Specifically, the immersed boundary-lattice Boltzmann method is utilized as the flow solver, and the stochastic receptor-ligand kinetics model is implemented to recover cell-substrate and cell-cell adhesive interactions. Special attention is paid to the roles of the cell deformability and adhesion strengths in cellular motion. Four distinct adhesion states, namely, rolling, tumbling, firm adhesion, and detachment, are identified and presented in phase diagrams as a function of the adhesion strengths for cell pairs with different deformabilities. The simulation results suggest that both the cell-cell and cell-substrate adhesion strengths act as the resistance to the rolling motion, and dominate the transition among various adhesion states. The cell deformability not only enhances the resistance effect, but also contributes to detachment or fast tumbling of the cell pair. These findings enrich the understanding of adhesion dynamics of cell aggregates, which could shed light on complex adhesion processes and provide instructions in developing adhesion-based applications.