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Prucalopride inhibits lung cancer cell proliferation, invasion, and migration through blocking of the PI3K/AKT/mTor

M Chen1, L-L Zhu1, J-L Su2

  • 1Department of Respiratory Medicine, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, People's Republic of China.

Human & Experimental Toxicology
|October 24, 2019
PubMed

Insights

Prucalopride, a 5-hydroxytryptamine 4 receptor agonist, was found to inhibit lung cancer progression. This study showed prucalopride reduced proliferation, invasion, and migration while inducing apoptosis in lung cancer cells.

Area of Science:

  • Oncology
  • Pharmacology
  • Molecular Biology

Background:

  • Lung cancer remains a leading global cause of cancer incidence and mortality.
  • The therapeutic potential of prucalopride, a 5-hydroxytryptamine 4 receptor agonist, in lung cancer treatment is not established.

Purpose of the Study:

  • To investigate the effects of prucalopride on lung cancer cell proliferation, invasion, migration, and apoptosis.
  • To elucidate the molecular mechanisms underlying prucalopride's action in lung cancer progression, focusing on the PI3K/AKT/mTor pathway.

Main Methods:

  • Cell Counting Kit 8 assay for proliferation.
  • Transwell assay for invasion and migration.
  • Flow cytometry and Western blot for apoptosis and protein expression analysis (PI3K/AKT/mTor pathway).

Main Results:

  • Prucalopride significantly inhibited proliferation, invasion, and migration of A549/A427 human lung cancer cells.
  • Prucalopride treatment induced autophagy and apoptosis in lung cancer cells.
  • Decreased expression of phosphorylated protein kinase B (AKT) and mammalian target of rapamycin (mTor) was observed.

Conclusions:

  • Prucalopride demonstrates an inhibitory role in the progression of human lung cancer.
  • The findings suggest prucalopride's potential as a therapeutic agent for lung cancer, mediated partly through the PI3K/AKT/mTor signaling pathway.
Keywords:
PI3K/AKTPrucaloprideautophagyinvasionlung cancermigrationproliferation

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