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Updated: Jan 5, 2026
Simultaneous ex vivo Functional Testing of Two Retinas by in vivo Electroretinogram System
Published on: May 6, 2015
Reproducibility of Fixed-luminance and Multi-luminance Flicker Electroretinography in Patients With Diabetic
John J Wroblewski1, Christa McChancy1, Kassandra Pickel2
1Cumberland Valley Retina Consultants, Hagerstown, MD, USA.
Background:
We evaluated the reproducibility of office-based flicker electroretinography (ERG) in patients with nonproliferative diabetic retinopathy (NPDR).
Methods:
An observational study was conducted in which ultra-widefield fluorescein angiography (UWF-FA) was performed on 20 patients with mild-to-moderate NPDR; images were graded by the Fundus Photography Reading Center (Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, WI, USA). Fixed- and multi-luminance flicker ERG was repeated four times (greater than or equal to seven days apart). Recording consistency was assessed using intra-class correlation coefficients (ICCs), coefficients of variation, and Pearson correlations.
Results:
82.5% and 17.5% of eyes had mild and moderate NPDR using UWF-FA; 90% of the angiograms were given a high confidence grade. Fixed-luminance phase values were highly reproducible (ICC: 0.949; P < .001). There was a significant negative correlation between fixed-luminance phase and log-corrected ischemic index values (-0.426; P = .015).
Conclusions:
Office-based, fixed-luminance phase values are highly reproducible and negatively correlate with retinal ischemia in NPDR, suggesting that global retinal dysfunction may be reliably quantified early in patients with diabetes.
