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Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice
Published on: June 22, 2016
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Annexin V expression on CD4+ T cells with regulatory function.
Anna-Lena Bollinger1, Thomas Bollinger2, Jan Rupp3
1Division of Immunology and Allergology, Department of Medicine, University Hospitals and Medical Faculty, Geneva, Switzerland.
Immunology
|October 24, 2019
Summary
Regulatory T (Treg) cells expressing Annexin V (ANXA5) were identified. These ANXA5+ Treg cells suppress immune responses and may bridge Treg function with immune silencing mechanisms.
Area of Science:
- Immunology
- Cell Biology
Background:
- Regulatory T (Treg) cells are crucial for inducing peripheral immunologic tolerance by suppressing effector lymphocytes.
- Immune silencing is primarily mediated by phagocyte recognition of phosphatidylserine (PS) on apoptotic cells.
Purpose of the Study:
- To investigate the expression and function of Annexin V (ANXA5) in regulatory T cells.
- To determine if ANXA5 expression links Treg function with immune silencing pathways.
Main Methods:
- Analysis of ANXA5 expression at mRNA and protein levels in CD4+ CD25hi Treg cells.
- In vitro functional assays including cell adhesion, proliferation, and suppression assays.
- In vivo studies using a murine model of delayed type hypersensitivity.
Main Results:
- CD4+ ANXA5+ T cells, a subset of Treg cells, express Treg markers and bind to PS+ cells.
- These cells exhibit potent, allo-specific suppression of T cell proliferation and inflammatory cytokine production.
- Murine CD4+ ANXA5+ T cells inhibit T helper type 1 responses in vivo.
Conclusions:
- This study reports Annexin V (ANXA5) expression on a novel subset of regulatory T cells.
- ANXA5+ Treg cells possess potent immunosuppressive capabilities.
- This finding suggests a potential role for ANXA5+ Treg cells in bridging classical Treg function with immune silencing.
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