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Circular RNA circC3P1 restrains kidney cancer cell activity by regulating miR-21/PTEN axis and inactivating PI3K/AKT

Tao Chen1, Qinchao Yu1, Lei Xin1

  • 1Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.

Journal of Cellular Physiology
|October 24, 2019
PubMed

Insights

Circular RNA C3P1 (circC3P1) inhibits kidney cancer progression by downregulating microRNA-21 (miR-21) and PTEN. This mechanism suppresses cell viability, migration, invasion, and apoptosis, impacting key signaling pathways.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Kidney cancer (KC) poses a significant public health challenge.
  • Understanding the molecular mechanisms underlying KC is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the function and mechanism of circular RNA C3P1 (circC3P1) in kidney cancer cells.
  • To elucidate the regulatory relationship between circC3P1, microRNA-21 (miR-21), and PTEN in KC.

Main Methods:

  • Cell viability, apoptosis, migration, and invasion assays (CCK-8, flow cytometry).
  • Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) for gene expression analysis.
  • Western blot analysis for protein expression and luciferase assay for interaction validation.

Main Results:

  • circC3P1 and PTEN were downregulated, while miR-21 was upregulated in KC tissues.
  • circC3P1 suppressed cell viability, migration, and invasion, and induced apoptosis in KC cells.
  • circC3P1 negatively regulated miR-21, and PTEN was identified as a direct target of miR-21.

Conclusions:

  • circC3P1 acts as a tumor suppressor in kidney cancer.
  • circC3P1 inhibits KC cell growth, migration, and invasion by regulating the miR-21/PTEN axis.
  • circC3P1 inactivates the PI3K/AKT and NF-κB signaling pathways, offering potential therapeutic targets.
Keywords:
circC3P1growthinvasionkidney cancermiR-21migration

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