Related Experiment Video
Updated: Jan 5, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Investigating the Function of Cryptic Cytochalasan Cytochrome P450 Monooxygenases Using Combinatorial Biosynthesis
Chongqing Wang1, Kevin Becker2, Sebastian Pfütze2
1Institute for Organic Chemistry and Centre for Biomolecular Drug Research , Leibniz University Hannover , Schneiderberg 38 , Hannover 30167 , Germany.
Abstract:
Tailoring enzymes in cytochalasan biosynthesis are relatively promiscuous. Exploiting this property, we deduced the function of four cryptic cytochrome P450 monooxygenases via heterologous expression of six cytochrome P450-encoding genes, originating from Hypoxylon fragiforme and Pyricularia oryzae, in pyrichalasin H ΔP450 strains. Three cryptic cytochrome P450 enzymes (HffD, HffG, and CYP1) restored pyrichalasin H production in mutant strains, while CYP3 catalyzed a site-selective epoxidation leading to the isolation of three novel cytochalasans.
Related Concept Videos
Biosynthesis in Bacteria
Peroxisomes
Drug Metabolism: Phase I Reactions
Biosynthesis of Lipids
Amino Acid Biosynthetic Pathways
Biosynthesis of Nucleic Acids
![Cercosporin-Photocatalyzed [4+1]- and [4+2]-Annulations of Azoalkenes Under Mild Conditions](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F60786.jpg&w=3840&q=50)
