Related Experiment Video
Updated: Jan 5, 2026
09:45
Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
Published on: February 9, 2012
25.8K
Wide-field time-correlated single photon counting-based fluorescence lifetime imaging microscopy
Klaus Suhling1, Liisa M Hirvonen1, Wolfgang Becker2
1Department of Physics, King's College London, Strand, London WC2R 2LS, UK.
Nuclear Instruments & Methods in Physics Research. Section A, Accelerators, Spectrometers, Detectors and Associated Equipment
|October 25, 2019
Summary
Wide-field time-correlated single photon counting (TCSPC) advances enable detailed fluorescence microscopy. These techniques capture photon arrival times and positions for enhanced imaging across various scientific fields.
Area of Science:
- Optics and Photonics
- Biophysics
- Microscopy Instrumentation
Background:
- Time-correlated single photon counting (TCSPC) is a powerful technique for precise timing measurements.
- Recent advancements have enabled wide-field TCSPC, simultaneously recording photon position and arrival time.
- Existing applications span nuclear science, mass spectroscopy, and positron emission tomography.
Purpose of the Study:
- To discuss recent advancements in wide-field TCSPC methods specifically for fluorescence microscopy.
- To highlight the application of these techniques in biological imaging.
- To present findings from the Ulitima fast imaging and tracking conference.
Main Methods:
- Utilizing wide-field cameras for simultaneous position and time recording of photons.
- Implementing TCSPC for high-resolution imaging.
- Applying the techniques to phosphorescence lifetime imaging (PLIM) and fluorescence lifetime imaging (FLIM).
Main Results:
- Demonstrated phosphorescence lifetime imaging (PLIM) on the microsecond timescale.
- Showcased fluorescence lifetime imaging (FLIM) on the nanosecond timescale.
- Highlighted diverse applications of wide-field TCSPC in fluorescence microscopy.
Conclusions:
- Wide-field TCSPC techniques offer significant advancements for fluorescence microscopy.
- These methods provide high temporal resolution for detailed biological imaging.
- The technology holds promise for various applications in life sciences and beyond.
