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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
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Colour compound lenses for a portable fluorescence microscope.

Bo Dai1, Ziao Jiao1, Lulu Zheng1

  • 11Engineering Research Center of Optical Instrument and System, the Ministry of Education, Shanghai Key Laboratory of Modern Optical System, University of Shanghai for Science and Technology, 200093 Shanghai, China.

Light, Science & Applications
|October 25, 2019
PubMed
Summary

A new handheld smartphone fluorescence microscope (HSFM) uses a unique dual-functional lens for imaging and filtering. This compact device enables point-of-care cellular analysis without bulky equipment.

Keywords:
Optical materials and structuresOptics and photonics

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Area of Science:

  • Biomedical Optics
  • Microscopy
  • Point-of-Care Diagnostics

Background:

  • Fluorescence imaging is crucial for detecting cells, proteins, and nucleic acids due to its high specificity and sensitivity.
  • Conventional fluorescence microscopes are bulky, limiting their use in point-of-care settings.
  • There is a need for portable, user-friendly fluorescence microscopy solutions.

Purpose of the Study:

  • To develop a compact, handheld smartphone fluorescence microscope (HSFM).
  • To integrate dual-functional polymer lenses for imaging and filtering, eliminating the need for separate optical filters.
  • To enable cellular-scale imaging and analysis using readily available smartphones for point-of-care applications.

Main Methods:

  • Designed and constructed a handheld smartphone fluorescence microscope (HSFM) integrating a smartphone, portable illumination, and a dual-functional polymer lens.
  • The polymer lens performs both optical imaging and light filtration, simplifying the microscope's design.
  • Tested the HSFM for various applications including cell and tissue observation, cell counting, and biological assays.

Main Results:

  • The HSFM achieved cellular-scale resolution for both bright-field and fluorescence imaging.
  • The device successfully performed cell observation, counting, plasmid transfection evaluation, and superoxide production analysis.
  • The dual-functional lens eliminated the need for additional optical filters, reducing device bulk and complexity.
  • The lens system demonstrated compatibility with various smartphone models.

Conclusions:

  • The developed HSFM offers a multifunctional, miniature fluorescence module for smartphones.
  • This technology simplifies fluorescence microscopy, making it suitable for real-time point-of-care diagnosis.
  • The HSFM has significant potential for personalized medicine and widespread clinical diagnostics.