P2X Electrophysiology and Surface Trafficking in Xenopus Oocytes
Eléonore Bertin1, Audrey Martínez1, Eric Boué-Grabot2
1Institut des Maladies Neurodégénératives, CNRS UMR 5293, Université de Bordeaux, Bordeaux, France.
Methods in Molecular Biology (Clifton, N.J.)
|October 25, 2019
Abstract:
Xenopus oocytes serve as a standard heterologous expression system for the study of various ligand-gated ion channels including ATP P2X receptors. Here we describe the whole-cell two-electrode voltage clamp and biotinylation/Western blotting techniques to investigate the functional properties and surface trafficking from P2X-expressing oocytes.
Keywords:
ATP-gated channelsDouble-electrode patch clampElectrophysiologyOocytesP2XReceptor functionSurface traffickingXenopus
