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Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

2.2K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.2K
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

8.1K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
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Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
1.5K
Mass Spectrometers01:16

Mass Spectrometers

8.1K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
8.1K
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

6.4K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

2.3K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
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Related Experiment Video

Updated: Jan 5, 2026

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
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Native mass spectrometry: a powerful tool for structural biology?

Tristan Free1

  • 1Future Science Group, Unitec House, 2 Albert Place, London, N3 1QB, UK.

Biotechniques
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PubMed
Summary

Mass spectrometry is a vital analytical research tool. This feature highlights its newest applications in various scientific fields.

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Environmental Science

Background:

  • Mass spectrometry (MS) is a long-established analytical technique.
  • It remains crucial for identifying and quantifying chemical substances.
  • Its versatility supports diverse research areas.

Discussion:

  • This feature examines cutting-edge uses of mass spectrometry.
  • It covers advancements in instrumentation and data analysis.
  • Emerging applications are explored in detail.

Key Insights:

  • Mass spectrometry continues to evolve with new technologies.
  • Its applications span multiple scientific disciplines.
  • Recent developments enhance sensitivity and scope.

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Analyzing Large Protein Complexes by Structural Mass Spectrometry
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Related Experiment Videos

Last Updated: Jan 5, 2026

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
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Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

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Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
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Outlook:

  • Future research will likely leverage advanced MS techniques.
  • Expect further integration into fields like personalized medicine.
  • Novel applications will continue to emerge.