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Related Experiment Video

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Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
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Massively-Parallelized, Deterministic Mechanoporation for Intracellular Delivery.

Harish G Dixit1, Renate Starr2, Morgan L Dundon3

  • 1Department of Bioengineering , University of California - Riverside , Riverside , California 92521 , United States.

Nano Letters
|October 25, 2019
PubMed
Summary
This summary is machine-generated.

Deterministic mechanoporation (DMP) offers a novel microfluidic solution for cellular engineering, overcoming the efficiency-viability trade-off. This method enhances intracellular delivery for improved cell product manufacturing.

Keywords:
Intracellular deliverycellular biomanufacturingcellular engineeringex vivo cell therapymechanoporationtransfection

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • Conventional cellular engineering faces limitations in delivery efficiency and cell viability.
  • Existing microfluidic poration methods often exhibit stochasticity, hindering optimal outcomes.

Purpose of the Study:

  • To introduce a novel microfluidic device for deterministic mechanoporation (DMP) of cells.
  • To address the trade-off between delivery efficiency and cellular viability in intracellular delivery.

Main Methods:

  • Aspiration-based cell capture followed by impingement on a needle-like penetrator for single-site poration.
  • Diffusive influx of exogenous cargo through the membrane pore upon flow reversal.
  • Massive parallelization for high-throughput operation.

Main Results:

  • DMP enables deterministic plasma membrane poration en masse, mitigating the efficiency-viability trade-off.
  • High efficiencies and viabilities achieved for delivering small and large molecules.
  • Exceeds performance of conventional and emerging transfection techniques, particularly in difficult-to-transfect cells.

Conclusions:

  • DMP is a promising microfluidic approach for advancing cellular engineering.
  • This technique shows significant potential for improving engineered cell product manufacturing.