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Normal and cancer cells response on the thin films based on chitosan and tannic acid
Beata Kaczmarek1, Oliwia Miłek2, Kinga Nadolna1
1Faculty of Chemistry, Department of Chemistry of Biomaterials and Cosmetics, Nicolaus Copernicus University in Toruń, Gagarin 7, 87-100 Toruń, Poland.
Abstract:
A novel aspect of tissue engineering is the material selective influence on the different cell types. The cell viability is a parameter which determine the cell ability to proliferate in the contact with material. In the experimental study the thin films based on chitosan and tannic acid mixture in ratio 80/20, 50/50 and 20/80 were tested. The surface roughness decreases with increasing tannic acid content. The cell culture was established on the proposed films. In vitro tests were carried out for the different cell lines as MNT-1, SK-MEL-28, Saos-2, HaCaT and BMSC. The result showed the dependence on the material influence on the various cell lines.
Insights
This study explores how chitosan and tannic acid films affect cell viability and proliferation. Material composition significantly influences different cell lines in tissue engineering applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Cell Biology
Background:
- Material properties critically influence cell behavior in tissue engineering.
- Cell viability and proliferation are key indicators of material biocompatibility.
- Chitosan and tannic acid are promising biomaterials for regenerative medicine.
Purpose of the Study:
- To investigate the material-selective influence of chitosan-tannic acid films on various cell types.
- To evaluate the effect of different chitosan/tannic acid ratios on surface roughness and cell viability.
- To assess the in vitro performance of these films with diverse cell lines.
Main Methods:
- Fabrication of thin films with varying chitosan/tannic acid ratios (80/20, 50/50, 20/80).
- Characterization of surface roughness in relation to tannic acid content.
- In vitro cell culture experiments using MNT-1, SK-MEL-28, Saos-2, HaCaT, and bone marrow-derived mesenchymal stem cells (BMSC).
Main Results:
- Surface roughness of the films decreased with increasing tannic acid content.
- Significant differences in cell viability and proliferation were observed across different cell lines when cultured on the films.
- The material composition demonstrated a selective influence on the various cell types tested.
Conclusions:
- Chitosan-tannic acid films exhibit material-selective effects on cell behavior.
- The ratio of chitosan to tannic acid influences film properties and cellular responses.
- These findings are crucial for designing tailored biomaterials for specific tissue engineering applications.
