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Melatonin treatment affects the glucoraphanin-sulforaphane system in postharvest fresh-cut broccoli (Brassica
Liyang Wei1, Changhong Liu1, Huanhuan Zheng1
1School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China.
Abstract:
The effect of postharvest melatonin treatment on sulforaphane production of fresh-cut broccoli at 4℃ during storage was investigated in this study. Florets treated with 100 μM melatonin exhibited higher contents of total glucosinolates and sulforaphane. Glucoraphanin content was significantly increased after melatonin treatment, and which was explained by gene analysis. Expressions of glucoraphanin biosynthesis genes including Elong, CYP83A1, MYB28, UGT74B1 and FMOGS-OX1 were up-regulated while AOP2 was obviously decreased by melatonin treatment, leading to a higher glucoraphanin accumulation. In addition, application of melatonin enhanced the myrosinase activity and the expression level of MYO, benefiting the formation of sulforaphane. This study demonstrates that melatonin treatment positively affected the glucoraphanin-sulforaphane system in postharvest fresh-cut broccoli.
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