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Updated: Jan 5, 2026
Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility
Published on: March 11, 2022
Double deletion of murA and murB induced temperature sensitivity in Corynebacterium glutamicum
Tuo Shi1,2, Qian Ma1,2,3,4, Xiaoqian Liu3
1Key Laboratory of Industrial Fermentation Microbiology, Tianjin University of Science & Technology, Ministry of Education, Tianjin, P. R. China.
Abstract:
Currently, the mechanism of temperature-sensitive production of glutamate in Corynebacterium glutamicum has not been clarified. We first found the murA and murB genes were potentially related to temperature-sensitive secretion of glutamate, which were not existed in a temperature-sensitive mutant. When replenishing murA or/and murB in the mutant, the temperature sensitivity was weakened. While, their knockout in a wild-type strain resulted in temperature-sensitive secretion of glutamate. Peptidoglycan analysis showed that deletion of murA and murB decreased the peptidoglycan synthesis. Comparative metabolomics analysis suggested that the variation in cell wall structure resulted in decreased overall cellular metabolism but increased carbon flow to glutamate synthesis, which was a typical metabolism pattern in industrial temperature-sensitive producing strains. This study clarifies the mechanism between murA and murB deletion and the temperature-sensitive secretion of glutamate in C. glutamcium, and provides a reference for the metabolic engineering of cell wall to obtain increased bioproduction of chemicals.
Insights
The study identifies murA and murB genes as key to temperature-sensitive glutamate production in Corynebacterium glutamicum. Deleting these genes alters cell walls, boosting glutamate synthesis for industrial applications.
Area of Science:
- Microbiology
- Metabolic Engineering
- Biochemistry
Background:
- The mechanism behind temperature-sensitive glutamate production in Corynebacterium glutamicum remains unclear.
- Understanding this mechanism is crucial for optimizing industrial bioproduction.
Purpose of the Study:
- To elucidate the role of specific genes in the temperature-sensitive secretion of glutamate in C. glutamicum.
- To investigate the link between cell wall synthesis and glutamate production.
Main Methods:
- Genetic manipulation: identifying and characterizing murA and murB genes in temperature-sensitive mutants and wild-type strains.
- Peptidoglycan analysis to assess cell wall synthesis.
- Comparative metabolomics to analyze metabolic shifts.
Main Results:
- The murA and murB genes were found to be crucial for temperature-sensitive glutamate secretion.
- Deletion of murA and murB in wild-type strains induced temperature sensitivity.
- Reduced peptidoglycan synthesis due to murA/murB deletion led to increased carbon flow towards glutamate synthesis.
Conclusions:
- The study clarifies the mechanism by which murA and murB influence temperature-sensitive glutamate secretion in C. glutamicum.
- Metabolic engineering of the cell wall presents a viable strategy for enhancing bioproduction of chemicals.
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