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Updated: Jan 5, 2026

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth
Published on: May 17, 2024
Stem Cells from Human Exfoliated Deciduous Teeth: A Concise Review
Chih-Sheng Ko1, Jen-Hao Chen2,3, Wen-Ta Su4
1PhytoHealth Corporation, Taipei, Taiwan.
Abstract:
Stem Cells from Human Exfoliated Deciduous Teeth (SHED) originate from the embryonic neural crest as ectodermal mesenchymal stem cells and are isolated from human deciduous teeth. SHED expresses the same cell markers as Embryonic Stem Cells (ESCs), such as OCT4 and NANOG, which make SHED to have a significant impact on clinical applications. SHED possess higher rates of proliferation, higher telomerase activity, increased cell population doubling, form sphere-like clusters, and possess immature and multi-differentiation capacity; such high plasticity makes SHED one of the most popular sources of stem cells for biomedical engineering. In this review, we describe the isolation and banking method, the current development of SHED in regenerative medicine and tissue engineering in vitro and in vivo.
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