Related Experiment Video
Updated: Jan 5, 2026
08:21
Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation
Published on: April 29, 2015
37.8K
Quantitation of TLR4 Internalization in Response to LPS in Thioglycollate Elicited Peritoneal mouse Macrophages by
Katharina Richard1, Stefanie N Vogel1, Darren J Perkins1
1Department of Microbiology and Immunology, University of Maryland Baltimore, Baltimore, USA.
Bio-Protocol
|October 26, 2019
Abstract:
Internalization of cell surface Toll Like Receptor 4 (TLR4) is a convenient read-out to measure LPS dependent activation of the TRIF adaptor pathway. We here provide a protocol to quantify the LPS dependent internalization of TLR4 using thioglycollate-elicited peritoneal macrophages by flow cytometry.
