Electrochemical lead(II) biosensor by using an ion-dependent split DNAzyme and a template-free DNA extension reaction...

A voltammetric biosensor for lead(II) (Pb²⁺) is described that is based on signal amplification by using an ion-dependent split DNAzyme and template-free DNA extension reaction. The Pb²⁺-dependent split DNAzyme was assembled on gold nanoparticles (Au@Fe₃O₄), and this nanoprobe then was exposed to Pb²⁺ which causes the split-off of DNAzymes to release primers containing 3'-OH groups (S₁ and S₂). The template-free DNA extension reaction triggers the generation of long ssDNA nanotails, which then can bind the free redox probe N,N'-bis(2-(trimethylammonium iodide)propylene)perylene-3,4,9,10-tetracarboxyldiimide (PDA⁺) via electrostatic adsorption. Hence, the concentration of PDA⁺ in solution is reduced. Therefore, less free PDA⁺ can be immobilized on a glassy carbon electrode modified with electrodeposited gold nanoparticles (depAu) to produce an electrochemical signal, typically measured at ∼0.38 V (vs. SCE) for quantitation of Pb²⁺. The use of a Pb²⁺-dependent split DNAzyme avoids the usage of a proteinic enzyme. It also increases the sensitivity of the sensor which has a lower detection limit of 30 pM of Pb²⁺. Graphical abstract Novel electrochemical biosensor based on the amplification of ion-dependent split DNAzyme and template-free DNA extension reaction for trace detection of Pb²⁺.