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Norepinephrine modulates the growth-inhibitory effect of transforming growth factor-beta in primary rat hepatocyte
K A Houck1, J L Cruise, G Michalopoulos
1Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710.
Abstract:
TGF-beta is a potent inhibitor of EGF-induced DNA synthesis in primary rat hepatocyte cultures. Norepinephrine (NE) was shown to modulate this inhibition of DNA synthesis. It produced a five-fold increase, from 2.8 pM to 14.4 pM, in the ID50 for TGF-beta. The effect was dose-dependent and was significant at concentrations of 10(-6)M NE and greater. The modulation by NE was mediated by the alpha 1-adrenergic receptor as shown by the ability of the alpha 1 antagonist prazosin to block the activity. This effect might be important during liver regeneration in allowing escape of hepatocytes from negative growth control exerted by TGF-beta.