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Updated: Jan 5, 2026
Methods for Acute and Subacute Murine Hindlimb Ischemia
Published on: June 21, 2016
Evaluation of skeletal muscle perfusion in a canine hind limb ischemia model using CT perfusion imaging
Tao Wang1, Haobo Su2, Jianping Gu2
1Department of Interventional Radiology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China;Department of Diagnostic Radiology, City of Hope National Medical Center, Duarte, CA, United States.
Purpose:
To evaluate skeletal muscle perfusion in a canine hind limb ischemia model using CT perfusion imaging (CTPI).
Methods:
Twelve beagles underwent embolization at the branch of the left deep femoral artery. The right hind limbs were used as controls. CTPI was performed immediately after embolization. The perfusion parameters of the regions of interest (ROI), including blood volume (BV), blood flow (BF), mean transit time (MTT) and permeability (PMB), were obtained in both the lateral and posterior hind limb muscle groups.
Results:
After embolization, the BV, BF and PMB values in the lateral muscles of the left hind limbs were significantly lower than those in the right hind limbs (P > 0.05), and the MTT was significantly prolonged (P > 0.05). The values for BV, BF, MTT and PMB in the posterior muscles of the left hind limbs were not significantly different from those in the right hind limbs (P > 0.05). The values for BV, BF and PMB in the lateral muscles of the left hind limbs were significantly lower than those in the posterior muscles of the left hind limbs (P > 0.05).
Conclusion:
CTPI could be used to evaluate skeletal muscle perfusion in a canine model, which may have clinical relevance in lower limb ischemia and vascular reconstruction.
