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Two distinct nucleic acid binding surfaces of Cdc5 regulate development.

Chao Wang1, Mu Li2, Guorui Li1

  • 1Key Laboratory of Pest Monitoring and Green Management, Ministry of Agriculture and Rural Affairs, Joint Laboratory for International Cooperationin Crop Molecular Breeding, Ministry of Education, and Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China.

The Biochemical Journal
|October 26, 2019
PubMed
Summary
This summary is machine-generated.

Cell division cycle 5 (Cdc5) protein uses distinct DNA and RNA binding surfaces for development regulation. Key residues like K100 and R31 are crucial for its nucleic acid binding and function in Arabidopsis thaliana.

Keywords:
DNA-binding domainMyb domainRNA bindingcrystalprotein structurerice blast fungus

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Area of Science:

  • Molecular Biology
  • Structural Biology
  • Developmental Biology

Background:

  • Cell division cycle 5 (Cdc5) is a conserved eukaryotic protein vital for development.
  • Cdc5's N-terminal DNA-binding domain (DBD) interacts with both DNA and RNA, but mechanisms are unclear.
  • Understanding Cdc5's nucleic acid binding is key to its regulatory roles in development.

Purpose of the Study:

  • Determine the molecular mechanism of Cdc5's nucleic acid recognition.
  • Elucidate how Cdc5 regulates development via nucleic acid binding.
  • Present the crystal structure of the MoCdc5-DBD.

Main Methods:

  • X-ray crystallography to determine the structure of MoCdc5-DBD.
  • Site-directed mutagenesis to investigate key residue functions (K100, R31).
  • Biochemical assays to assess nucleic acid binding affinity.

Main Results:

  • Crystal structure of MoCdc5-DBD reveals a conserved positively charged groove.
  • Mutation of K100 affects binding to Cdc5-specific elements but not Myb elements.
  • Mutation of R31 significantly reduces U6 RNA binding.
  • Mutations in key residues of Arabidopsis AtCDC5 cause developmental defects and affect microRNA levels.

Conclusions:

  • MoCdc5-DBD utilizes distinct binding surfaces for DNA and RNA.
  • Key residues K100 and R31 are critical for specific nucleic acid interactions.
  • Cdc5's nucleic acid binding is essential for regulating eukaryotic development.