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Published on: March 30, 2022
Role of matrix metalloproteases 1/3 gene polymorphisms in patients with rotator cuff tear
Kaisong Miao1, Lifeng Jiang2, Xindie Zhou1
1Department of Orthopedics, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, Changzhou 213000, China.
Abstract:
An association of Matrix Metalloproteinases-1/3 (MMP-1/3) rs1799750/rs3025058 polymorphism with increased risk of rotator cuff tear (RCT) has been reported in a Brazilian population. However, this significant association has not been confirmed in the Chinese population. Genotyping was conducted by polymerase chain reaction (PCR)-restriction fragment length polymorphism and direct sequencing. Our results demonstrated that individuals with the TT genotype had a significantly higher risk of RCT compared with those with the CC genotype. The increased risk of RCT progression was associated with the 2G allele of the rs1799750 polymorphism. No significant association was observed for genotypic and allelic frequencies of the rs3025058 polymorphism. A significant association of the MMP-1 rs1799750 polymorphism was observed with smokers, drinkers and people aged ≥60 years and non-diabetic people. Additionally, the MMP-1 rs1799750 polymorphism was associated with pre-operative stiffness in RCT patients. In conclusion, a significant correlation was identified between the MMP-1 rs1799750 polymorphism and RCT. The MMP-1 rs1799750 polymorphism might be considered as a biomarker of genetically high-risk RCT, helping to clarify the mechanism of RCT.
Insights
The Matrix Metalloproteinases-1 (MMP-1) rs1799750 polymorphism is linked to a higher risk of rotator cuff tears (RCT). This genetic marker may help identify individuals predisposed to RCT, aiding in understanding its mechanisms.
Area of Science:
- Genetics
- Orthopedics
- Biomarkers
Background:
- Previous studies suggested a link between Matrix Metalloproteinases-1/3 (MMP-1/3) rs1799750/rs3025058 polymorphisms and rotator cuff tear (RCT) risk in Brazilians.
- This association remained unconfirmed in the Chinese population, necessitating further investigation.
Purpose of the Study:
- To investigate the association between MMP-1/3 rs1799750/rs3025058 polymorphisms and the risk of rotator cuff tear (RCT) in the Chinese population.
- To explore potential correlations between the MMP-1 rs1799750 polymorphism and clinical factors in RCT patients.
Main Methods:
- Genotyping was performed using polymerase chain reaction (PCR)-restriction fragment length polymorphism and direct sequencing.
- Allelic and genotypic frequencies of MMP-1/3 rs1799750/rs3025058 polymorphisms were analyzed in relation to RCT risk.
- Associations with demographic factors (age, smoking, drinking, diabetes) and clinical presentation (pre-operative stiffness) were assessed.
Main Results:
- The TT genotype of the MMP-1 rs1799750 polymorphism was significantly associated with an increased risk of RCT compared to the CC genotype.
- The 2G allele of MMP-1 rs1799750 was linked to a higher risk of RCT progression.
- No significant association was found for the MMP-1/3 rs3025058 polymorphism.
- MMP-1 rs1799750 showed significant associations with smokers, drinkers, individuals aged ≥60 years, non-diabetic individuals, and pre-operative stiffness in RCT patients.
Conclusions:
- The MMP-1 rs1799750 polymorphism is significantly correlated with rotator cuff tear (RCT) risk in the Chinese population.
- This polymorphism may serve as a potential biomarker for genetically high-risk individuals susceptible to RCT.
- Understanding this genetic link can contribute to elucidating the underlying mechanisms of RCT development.
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