Related Experiment Video

Updated: Jan 5, 2026

Author Spotlight: A Selective Luciferase-Based Assay for Monitoring ATG4B 27 Activity in Cells
09:51

Author Spotlight: A Selective Luciferase-Based Assay for Monitoring ATG4B 27 Activity in Cells

Published on: June 30, 2023

1.4K

USP14 Inhibition Regulates Tumorigenesis by Inducing Autophagy in Lung Cancer In Vitro

Kyung Ho Han1, Minseok Kwak2, Tae Hyeong Lee3

  • 1Department of Biomedical Sciences, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea. Kyungho1.han@gmail.com.

International Journal of Molecular Sciences
|October 27, 2019
PubMed

Insights

Ubiquitin-specific protease 14 (USP14) is highly expressed in lung cancer and drives tumor growth. Inhibiting USP14 significantly reduced lung cancer cell proliferation and invasion, suggesting its potential as a therapeutic target.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • The ubiquitin-proteasome system regulates cellular pathways crucial for oncogenesis.
  • Deubiquitination, mediated by enzymes like ubiquitin-specific protease 14 (USP14), negatively impacts cancer-associated substrates.
  • USP14 is implicated in various cancers, but its specific role in lung tumorigenesis requires further elucidation.

Purpose of the Study:

  • To investigate the expression and function of USP14 in lung cancer.
  • To evaluate the therapeutic potential of USP14 inhibitors in lung cancer models.

Main Methods:

  • Quantitative analysis of USP14 expression in lung cancer patient samples.
  • In vitro studies using USP14 inhibitors (IU1-47) and genetic knockdown (siRNA-USP14) to assess effects on lung cancer cell lines.
  • Assessment of apoptosis and autophagy pathways in response to USP14 inhibition.

Main Results:

  • USP14 was found to be highly expressed in lung cancer patients.
  • USP14 inhibition significantly reduced lung cancer cell proliferation, migration, and invasion.
  • USP14 was shown to negatively regulate lung tumorigenesis via both apoptosis and autophagy pathways.

Conclusions:

  • USP14 plays a critical role in the development and progression of lung cancer.
  • USP14 inhibitors demonstrate significant anti-cancer activity and represent promising therapeutic agents for lung cancer treatment.
Keywords:
autophagydeubiquitinating enzymelung cancerubiquitin-specific protease 14

More Related Videos

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer06:51

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer

Published on: July 21, 2018

18.5K
The Soft Agar Colony Formation Assay08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

113.4K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.6K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.0K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
5.2K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
5.8K
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
8.1K