Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electrophoresis: Overview01:20

Electrophoresis: Overview

3.5K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
3.5K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

1.0K
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
1.0K
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

848
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
848

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Harnessing CRISPR-Cas12 and Microfluidics Chips for Multiplex Respiratory Pathogens Diagnosis.

ACS sensors·2026
Same author

Smartphone-Based Microbubble-Linked Immunosorbent Assay Powered by Classification-Regression Integrated Deep Learning for Portable Quantitative Biomarker Analysis.

ACS nano·2026
Same author

Sulfated bile acid produced by a human gut commensal alleviates paediatric sepsis in mice.

Nature microbiology·2026
Same author

Culture-Free Microfluidics for Ultra-Rapid Antimicrobial Susceptibility Testing with AI in Resource-Limited Settings.

Analytical chemistry·2026
Same author

High-throughput identification of endogenous biomolecular condensates and phase-separating proteins.

Nature protocols·2026
Same author

Self-Powered Gravity Domino Microfluidics for Rapid At-Home Immunoassays with Artificial Intelligence.

ACS nano·2026

Related Experiment Video

Updated: Jan 5, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

15.4K

Microfluidic chip electrophoresis for biochemical analysis.

Xiaowen Ou1,2, Peng Chen2, Xizhi Huang2

  • 1Hubei Key Laboratory of Purification and Application of Plant Anti-Cancer Active Ingredients, College of Chemistry and Life Science, Hubei University of Education, Wuhan, P. R. China.

Journal of Separation Science
|October 27, 2019
PubMed
Summary
This summary is machine-generated.

Microfluidic chip electrophoresis offers efficient separation of biochemicals with low sample use and high throughput. This review covers its modes, detection methods, and applications in analyzing biomacromolecules, small molecules, and bioparticles.

Keywords:
analysisbiochemistryelectrophoresismicrofluidic chips

More Related Videos

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

17.8K
Microfluidic Chip Fabrication and Method to Detect Influenza
09:43

Microfluidic Chip Fabrication and Method to Detect Influenza

Published on: March 26, 2013

15.4K

Related Experiment Videos

Last Updated: Jan 5, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

15.4K
A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

17.8K
Microfluidic Chip Fabrication and Method to Detect Influenza
09:43

Microfluidic Chip Fabrication and Method to Detect Influenza

Published on: March 26, 2013

15.4K

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Microfluidics

Background:

  • Microfluidic chip electrophoresis (MCE) is a powerful technique for biochemical separation.
  • It offers advantages like low sample consumption, cost-effectiveness, rapid analysis, and high throughput.
  • Its integration capability allows for complex analytical workflows on a single chip.

Purpose of the Study:

  • To review the development of different microfluidics-based electrophoresis technologies.
  • To discuss various detection schemes coupled with microfluidic electrophoresis platforms.
  • To explore innovative applications of MCE in analyzing diverse biological samples.

Main Methods:

  • Review of four microfluidics-based electrophoresis modes: capillary electrophoresis, gel electrophoresis, dielectrophoresis, and field (electric) flow fractionation.
  • Examination of coupled detection schemes: optical, electrochemical, and mass spectrometry.
  • Analysis of applications in biomacromolecules, small molecules, and bioparticles.

Main Results:

  • Detailed overview of the advancements in microfluidic electrophoresis technologies.
  • Comprehensive summary of integrated detection methods for enhanced analysis.
  • Demonstration of MCE's versatility in analyzing nucleic acids, proteins, metabolites, ions, cells, and pathogens.

Conclusions:

  • Microfluidic chip electrophoresis is a versatile and evolving technology for biochemical analysis.
  • Continued development promises further advancements in sensitivity, throughput, and application scope.
  • Future directions point towards enhanced capabilities for complex biological sample analysis.