Related Experiment Video
Updated: Jan 5, 2026
Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR
Published on: February 12, 2018
Direct quantification of intact FIM in monkey plasma using a selective chromatography-tandem mass spectrometry
Shiqi Dong1, Aijie Zhang1, Yuan Gu2
1Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China.
Abstract:
FIM protein, which consists of 155 amino acids, was developed as a novel GLP-1 analog to reduce blood glucose, and pharmacodynamic results showed that it had a certain effect when used in treating Alzheimer's disease. The molecular weight of FIM is 16,304 Da. In theory, the concentration of FIM in biological samples should be determined by the ligand binding assay method or indirectly quantified using LC-MS/MS instrumentation. However, the above methods are complex and time-consuming. In this study, we successfully developed a simpler LC-MS/MS method for directly quantifying the intact FIM protein in monkey plasma for the first time. The chromatographic separation of FIM was achieved using an InertSustain Bio C18 column with a mobile phase of acetonitrile containing 0.1% formic acid (A)-water containing 0.1% formic acid (B) at a flow rate of 0.3 ml/min. Good linearity was observed in the concentration range of 5-500 ng/ml (r2 > 0.99). The intra- and inter-day precisions (expressed as relative standard deviation, RSD) of FIM were 2.30-12.8 and 7.30-13.2%, respectively. The intra- and inter-day accuracies (expressed as a relative error, RE) were -12.7-6.55 and - 10.1-0.892%, respectively. This method was successfully applied for a pharmacokinetic study of the FIM protein in four monkeys after subcutaneous administration.
Insights
A new, simpler LC-MS/MS method was developed to quantify the FIM protein in monkey plasma. This method enables direct measurement of the novel GLP-1 analog, aiding in its pharmacokinetic studies.
Area of Science:
- Pharmacology
- Analytical Chemistry
- Biochemistry
Background:
- FIM protein, a novel GLP-1 analog, shows potential in treating Alzheimer's disease.
- Existing methods for quantifying FIM protein in biological samples are complex and time-consuming.
Purpose of the Study:
- To develop a simpler and more efficient LC-MS/MS method for direct quantification of intact FIM protein in monkey plasma.
- To establish a reliable method for pharmacokinetic studies of FIM protein.
Main Methods:
- Development of a direct LC-MS/MS method for FIM protein quantification.
- Utilized an InertSustain Bio C18 column with a specific mobile phase (acetonitrile/water with formic acid).
- Validated the method for linearity, precision, and accuracy in monkey plasma.
Main Results:
- Achieved good linearity in the concentration range of 5-500 ng/ml (r² > 0.99).
- Demonstrated acceptable intra- and inter-day precision (RSD: 2.30-12.8% and 7.30-13.2%) and accuracy (RE: -12.7-6.55% and -10.1-0.892%).
- Successfully applied the method to a pharmacokinetic study in monkeys.
Conclusions:
- The developed LC-MS/MS method is simpler and more efficient for direct quantification of intact FIM protein.
- This method is suitable for pharmacokinetic analysis of FIM protein in biological matrices like monkey plasma.
More Related Videos
11:25Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
Published on: July 11, 2014
10:13Author Spotlight: An Efficient Methodology to Confidently Differentiate and Characterize Fentanyl Analogs
Published on: November 8, 2024
