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Related Experiment Video

Updated: Jan 5, 2026

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures
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Single-cell analysis for drug development using convex lens-induced confinement imaging.

Ndeye Khady Thiombane1, Nicolas Coutin1, Daniel Berard2

  • 1Pharmaceutical Sciences, 2405 Wesbrook Mall, University of British Columbia, Vancouver, BC, V6T1Z3, Canada.

Biotechniques
|October 29, 2019
PubMed
Summary

Convex lens-induced confinement (CLiC) microscopy offers high-quality single-cell imaging. This new method captures cellular heterogeneity for improved drug discovery and extended proliferation studies.

Keywords:
CLiCdose-responsemethotrexatephenotypic screensingle cellyeast

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Area of Science:

  • Life Sciences
  • Cellular Biology
  • Microscopy

Background:

  • Advanced life science technologies provide population-level data.
  • Existing methods mask single-cell heterogeneity, hindering drug discovery.
  • A novel single-cell imaging approach is needed.

Purpose of the Study:

  • To introduce and validate convex lens-induced confinement (CLiC) microscopy.
  • To demonstrate CLiC's capability for high-resolution single-cell analysis.
  • To assess CLiC's utility in drug response and proliferation studies.

Main Methods:

  • Development of convex lens-induced confinement (CLiC) microscopy.
  • Validation using yeast cells for subcellular localization.
  • Live-cell assays to monitor proliferation and drug response.

Main Results:

  • CLiC achieved enhanced signal-to-noise and sensitivity compared to traditional imaging.
  • Cellular proliferation times in CLiC assays matched flask culture standards.
  • Methotrexate treatment induced a fivefold increase in cell size, captured by CLiC.

Conclusions:

  • CLiC microscopy enables high-quality, single-cell imaging.
  • The method accurately tracks cellular drug responses and proliferation over extended periods.
  • CLiC advances single-cell analysis for drug discovery and development.