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Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats
Published on: December 7, 2021
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Pedobacter indicus sp. nov., isolated from deep-sea sediment
Xiao-Yan He1, Na Li1, Xiu-Lan Chen1,2
1State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao, 266237, China.
Antonie Van Leeuwenhoek
|October 30, 2019
Summary
A novel bacterium, Pedobacter indicus sp. nov. strain SM1810T, was discovered in deep-sea sediment. This research details its unique characteristics and phylogenetic placement within the Pedobacter genus.
Area of Science:
- Microbiology
- Marine Biology
- Bacterial Taxonomy
Background:
- Deep-sea environments harbor diverse microbial life.
- The genus Pedobacter comprises Gram-negative bacteria with various ecological roles.
- Characterization of novel bacterial species is crucial for understanding microbial diversity and evolution.
Purpose of the Study:
- To isolate and characterize a novel bacterium from deep-sea sediment.
- To determine the phylogenetic and taxonomic position of the novel isolate.
- To propose a new species within the genus Pedobacter.
Main Methods:
- Isolation and cultivation of bacteria from deep-sea sediment.
- Gram staining, aerobic cultivation, and morphological analysis.
- Phylogenetic analysis based on 16S rRNA gene sequencing.
- Chemotaxonomic analysis including fatty acid composition and polar lipid profiling.
- Determination of genomic DNA G+C content.
Main Results:
- A novel Gram-negative, aerobic, rod-shaped bacterium, strain SM1810T, was isolated.
- Phylogenetic analysis showed affiliation with the genus Pedobacter, with 95.8% 16S rRNA gene sequence similarity to Pedobacter bauzanensis.
- Key chemotaxonomic features include major fatty acids iso-C15:0, summed feature 3, and iso-C17:0 3OH, and MK-7 as the predominant respiratory quinone.
- The genomic DNA G+C content was 40.8 mol% and growth occurred between 15-40 °C, pH 5.0-9.0, and 0-8% NaCl.
Conclusions:
- Strain SM1810T represents a novel species of the genus Pedobacter.
- The proposed name for this new species is Pedobacter indicus sp. nov.
- The type strain is SM1810T (KCTC 62798T = CCTCC AB 2018198T).
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