Perylene diimide as a cathodic electrochemiluminescence luminophore for immunoassays at low potentials

In the cathodic electrochemiluminescence (ECL) field, most reported luminophores produced ECL emission at high potentials (more than -1.3 V vs. Ag/AgCl), which was adverse for both fundamental studies and practical application. It was important to screen novel ECL luminophores and coreactants for the development of ECL. In this work, N,N'-dimethyl-3,4,9,10-perylenedicarboximide (PDI-CH₃) is reported to produce ECL at -0.47 V using K₂S₂O₈ as a coreactant in an aqueous system. In addition, the ECL wavelength was 689 nm, which was interpreted with the emission of excited PDI-CH₃ dimers. Finally, this low-triggering-potential ECL system was used to construct sandwiched immunosensors to detect carcinoembryonic antigen (CEA) with the potential range from 0 to -0.8 V. In this immunosensor, PDI-CH₃ and gold nanoparticles (AuNPs) reduced by citrate were grafted onto graphite oxide (GO) to label secondary antibodies (Ab₂). This immunosensor could sensitively detect CEA with the linear response range between 1 fg mL^-1 and 1 μg mL^-1 and detection limit 0.29 fg mL^-1. In addition, this immunosensor showed good feasibility in various cancer serum samples.