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Published on: November 4, 2014
Characterization and pathogenicity of Vero cell-attenuated porcine epidemic diarrhea virus CT strain
Yu Wu1, Wei Li2, Qingfeng Zhou2
1College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou, 510642, People's Republic of China.
Background:
Porcine epidemic diarrhea virus (PEDV) has caused enormous economic losses to the global pig industry. Currently available PEDV vaccine strains have limited protective effects against PEDV variant strains.
Methods:
In this study, the highly virulent epidemic virus strain CT was serially passaged in Vero cells for up to 120 generations (P120). Characterization of the different passages revealed that compared with P10 and P64, P120 had a higher viral titer and more obvious cytopathic effects, thereby demonstrating better cell adaptability.
Results:
Pathogenicity experiments using P120 in piglets revealed significant reductions in clinical symptoms, histopathological lesions, and intestinal PEDV antigen distribution; the piglet survival rate in the P120 group was 100%. Furthermore, whole-genome sequencing identified 13 amino acid changes in P120, which might be responsible for the attenuated virulence of P120.
Conclusions:
Thus, an attenuated strain was obtained via cell passaging and that this strain could be used in preparing attenuated vaccines.
