Hyunsoonleella flava sp. nov., isolated from marine sediment

A novel Gram-stain-negative, strictly aerobic, rod-shaped, non-motile and yellow-pigmented bacterial strain, designated T58^T, was isolated from a marine sediment sample collected from the coastal area of Weihai, PR China. Strain T58^T was most closely related to Hyunsoonleella pacifica SW033^T with 97.1 % 16S rRNA gene sequence similarity, followed by Hyunsoonleella jejuensis KCTC 22242^T (96.9 %). Based on 16S rRNA gene sequences, the phylogenetic analysis indicated that strain T58^T represented a member of the genus Hyunsoonleella within the family Flavobacteriaceae of the phylum Bacteroidetes. Strain T58^T was found to grow optimally at 30 °C, at pH 7.0-7.5 and in the presence of 2.0-3.0 % (w/v) NaCl. The major fatty acids were iso-C_{15  :  0}, iso-C_15 : 1 G, iso-C_17 : 0 3-OH and iso-C_15 : 0 3-OH. The major isoprenoid quinone was menaquinone 6 (MK-6). The major polar lipids were phosphatidylethanolamine, two unidentified aminolipids and two unidentified lipids. The DNA G+C content of the genomic DNA was 35.0 mol%. On the basis of its phylogenetic, phenotypic and chemotaxonomic characteristics, strain T58^T is considered to represent a novel species of the genus Hyunsoonleella, for which the name Hyunsoonleella flava sp. nov. is proposed. The type strain is T58^T (=KCTC 72081^T=MCCC 1H00359^T).