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Cumulus Cells Are Potential Candidates for Cell Therapy
Eslam Essam Mohammed1, Sema Yilmaz2, Oya Alagoz Akcin3
1Department of Biochemistry, Yildiz Technical University, Istanbul, Turkey.
Background/Aim:
Cumulus cells (CCs) originate from the membrane granulosa cells and surround oocytes during follicle maturation. CCs produce high levels of hyaluronan that targets CD44, which is a major tumorigenic marker. This study aimed to investigate whether CCs have a role in cell therapy by targeting CD44 in pancreatic cancer cells.
Materials And Methods:
CCs were isolated from the oocytes and incubated in a hypoxic environment. BxPC-3 pancreatic cancer cells were treated with CC conditioned media for three days.
Results:
Conditioned media of CC cells incubated in hypoxic conditions caused a 25% reduction in the viability of BxPC-3 cells. Expression of anti-apoptotic genes was down-regulated, while that of pro-apoptotic genes was upregulated. An increased number of BxPC-3 cells exhibited increased levels of reactive oxygen species and arrested in the synthesis (S) phase of the cell cycle.
Conclusion:
CCs conditioned medium induced apoptosis of pancreatic cancer cells.
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