Isolation and Genome Sequencing of a Novel Pseudomonas aeruginosa Phage PA-YS35
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Published on: April 9, 2015
Yun-He Jiang1, Jia-Qi Liu1, Chun-Yan Zhao1
1Department of Pathogenobiology, College of Basic Medical Science, Jilin University, Changchun, 130021, Jilin, China.
Abstract:
Phage PA-YS35 is a novel lytic Pseudomonas aeruginosa phage belonging to the Myoviridae family and was isolated from the sewage of the First Hospital of Jilin University. The biological properties testing indicated that phage PA-YS35 is stable between - 20 and 60 °C and pH 4-9. The one-step growth curve shows that the latent period of PA-YS35 was 9 min, and the burst period was about 21 min by the size of approximately 380 progeny phages per host cell. The genome of phage PA-YS35 is linear double-stranded DNA with a size of 93,296 bp and a GC content of 49.35%. The results from RAST gene annotation analysis showed that the PA-YS35 genome contains 172 open reading frames (ORFs); the function of 41 ORFs can be predicted, whereas the product of remaining 131 ORFs are hypothetical proteins. According to phylogenetic tree of RNA ligase encoding sequence, phage PA-YS35 has a close evolutionary relationship with Pseudomonas phage PAK P1 because both of them are located on the same branch. The study of phage PA-YS35 genome will provide useful information for further research on the interaction between phages and their hosts.
Insights
A novel Pseudomonas aeruginosa phage, PA-YS35, was isolated and characterized. This lytic phage exhibits stability across a range of temperatures and pH levels, offering potential for therapeutic applications.
Area of Science:
- Microbiology
- Virology
- Bacteriology
Background:
- Pseudomonas aeruginosa is an opportunistic pathogen causing significant hospital-acquired infections.
- Bacteriophages offer a promising alternative or adjunct to conventional antibiotics for treating bacterial infections.
Purpose of the Study:
- To isolate and characterize a novel lytic bacteriophage targeting Pseudomonas aeruginosa.
- To analyze the biological properties and genomic features of the isolated phage PA-YS35.
Main Methods:
- Isolation of phage PA-YS35 from hospital sewage.
- Biological property assessment (stability, growth curve).
- Genomic analysis (DNA sequencing, RAST annotation, phylogenetic analysis).
Main Results:
- Phage PA-YS35 is a Myoviridae family member, stable between -20 to 60°C and pH 4-9.
- One-step growth curve revealed a 9-min latent period and a 21-min burst period, yielding ~380 progeny phages.
- Genome is 93,296 bp linear double-stranded DNA with 49.35% GC content, containing 172 ORFs (41 with predicted functions).
- Phylogenetic analysis indicated a close relationship with Pseudomonas phage PAK P1.
Conclusions:
- Phage PA-YS35 is a robust lytic phage with potential for P. aeruginosa infection control.
- Genomic data provides insights into phage-host interactions and phage biology.
- Further research into PA-YS35 can advance phage therapy strategies.
