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A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Sinirhodobacter hankyongi sp. nov., a novel denitrifying bacterium isolated from sludge
Young Woo Lee1, Tran Son Hoang2, Moon-Soo Rhee3
1Department of Biotechnology, Hankyong National University, 327 Chungang-no Anseong-si, Gyeonggi-do 17579, Republic of Korea.
Abstract:
A novel Gram-reaction-negative, facultative-aerobic, motile, non-spore-forming, oval-shaped and denitrifying bacterium, designated BO-81T, was isolated from sludge sampled in the Republic of Korea. This bacterium was investigated via a polyphasic approach to reveal its taxonomic position. The results of phylogenetic analysis based on 16S rRNA gene sequencing indicated that strain BO-81T belonged to the family Rhodobacteraceae and was related to the closest species Sinirhodobacter ferrireducens (98.8 % sequence similarity), 'Sinorhodobacter hungdaonensis' (98.4 %), Rhodobacter lacus (97.8 %), Sinorhodobacter populi (96.8 %) and Rhodobacter maris (96.2 %). The average nucleotide identity and DNA-DNA hybridization values between strain BO-81T and its closely related strains were 77.7-94.2 % and 20.1-55.9 %, respectively, indicating that BO-81T represents a novel species of the genus Sinirhodobacter. Growth occurred at 18-40 °C on Reasoner's 2A medium in the presence of 0-7 % NaCl (w/v) and at pH 6.0-9.0. Strain BO-81T was characterized chemotaxonomically as having ubiquinone 10 as its predominant respiratory quinone, summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) as its major fatty acid and phosphatidylglycerol and phosphatidylethanolamine as its predominant polar lipids. The G+C content of the genomic DNA was 68.3 mol%. The results of physiological and biochemical tests allowed phenotypic differentiation of strain BO-81T from other Sinirhodobacter species with validly published names. Therefore, the isolate represented a novel species, for which the name Sinirhodobacter hankyongi sp. nov. (type strain BO-81T=KACC 19677T=LMG 30808T) is proposed.
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