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Characterization of Leukocyte-platelet Rich Fibrin, A Novel Biomaterial
Published on: September 29, 2015
In vitro human cord blood platelet lysate characterisation with potential application in wound healing
Paola Losi1, Maria C Barsotti1, Ilenia Foffa1
1Laboratorio di Medicina Rigenerativa, Biomateriali e terapie avanzate, Institute of Clinical Physiology, National Research Council, Massa, Italy.
Insights
Human umbilical cord blood platelet lysate (UCB-PL) promotes wound healing by enhancing cell migration and proliferation. This UCB-PL offers a standardized, effective regenerative tool for clinical applications.
Area of Science:
- Regenerative Medicine
- Cell Biology
- Biotechnology
Background:
- Platelets are rich in growth factors crucial for cell migration and proliferation.
- Human umbilical cord blood platelet lysate (UCB-PL) contains supraphysiological growth factor concentrations.
Purpose of the Study:
- To investigate the effectiveness of human UCB-PL in wound healing processes.
- To evaluate the impact of UCB-PL on various human and mouse cell types.
Main Methods:
- Human UCB-PL was prepared via freeze/thaw of platelet concentrate.
- UCB-PL effects were assessed on endothelial cells, monocytes, fibroblasts, and keratinocytes at varying concentrations.
- Pro-angiogenic growth factor levels were compared to peripheral blood platelet-rich plasma.
Main Results:
- UCB-PL exhibited higher pro-angiogenic factors than peripheral blood platelet-rich plasma.
- Optimal UCB-PL concentrations varied for cell viability and proliferation (e.g., 5% for L929, 10% for HUVEC).
- UCB-PL significantly enhanced monocyte migration and keratinocyte scratch closure.
Conclusions:
- Human UCB-PL demonstrates significant potential for wound healing applications.
- UCB-PL provides a standardized, scalable source of growth factors, minimizing inter-individual variability.
- UCB-PL can serve as a functional tool for clinical regenerative therapy.
Abstract:
Platelets contain abundant growth factors and cytokines that have a positive influence on the migration and proliferation of different cell types by modulating its physiopathological processes. As it is known that human umbilical cord blood platelet lysate (UCB-PL) contains a supraphysiological concentration of growth factors, in the present study, we investigated its effectiveness in wound-healing processes. Human UCB-PL was obtained by the freeze/thaw of platelet concentrate (1.1 × 109 platelets/L), and its effect was evaluated on human or mouse endothelial cells, monocytes, fibroblasts, and keratinocytes in different concentrations. Human UCB-PL was observed to have high levels of pro-angiogenic growth factor than peripheral blood platelet-rich plasma. Among the cell lines, different concentrations of human UCB-PL were necessary to influence their viability and proliferation. For L929 cells, 5% of total volume was necessary, while for human umbilical vein endothelial cell, it was 10%. Cell migration on monocytes was increased with respect to the positive control, and scratch closure on keratinocytes was increased with respect to serum-free medium with only 10% of human UCB-PL. We concluded that the human UCB-PL may be useful to produce a large amount of standard platelet concentrates sufficient for several clinical-scale expansions avoiding inter-individual variability, which can also be used as a functional tool for clinical regenerative application for wound healing.
