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A method for the simultaneous evaluation of exocrine pancreatic function and intestinal absorptive function in dogs
American Journal of Veterinary Research
|August 1, 1979
Summary
This study combined pancreatic function and small intestine absorption tests in dogs. Pancreatic duct ligation impaired exocrine function but did not affect xylose absorption, demonstrating a novel combined diagnostic approach.
Area of Science:
- Veterinary Medicine
- Gastroenterology
- Diagnostic Testing
Background:
- Exocrine pancreatic function is crucial for digestion.
- Evaluating pancreatic and intestinal function often requires separate tests.
- The BT-PABA assay measures pancreatic chymotrypsin activity.
- The xylose absorption test assesses small intestinal absorptive capacity.
Purpose of the Study:
- To develop and validate a combined diagnostic test for evaluating exocrine pancreatic function and small intestinal absorptive function in dogs.
- To assess the impact of pancreatic duct ligation on both digestive and absorptive functions.
Main Methods:
- A single oral solution containing N-benzoyl-L-tyrosyl-p-aminobenzoic acid (BT-PABA) and d(+)xylose was administered to dogs.
- Blood concentrations of p-aminobenzoic acid (PABA) and xylose were measured at intervals.
- The combined test was performed in control dogs and dogs with pancreatic duct ligation.
Main Results:
- Peak PABA concentrations in control dogs occurred between 60-120 minutes post-administration.
- Peak xylose concentrations in control dogs occurred between 30-90 minutes post-administration.
- Pancreatic duct ligation significantly reduced PABA levels (by one-sixth at 90 minutes), indicating impaired exocrine pancreatic function.
- Xylose absorption remained unaffected by pancreatic duct ligation.
Conclusions:
- The combined BT-PABA and xylose test effectively differentiates exocrine pancreatic dysfunction from impaired intestinal absorption in dogs.
- This novel, single 90-minute test provides a valuable tool for diagnosing gastrointestinal disorders in canine patients.
- Pancreatic exocrine function is distinct from small intestinal absorptive function, as demonstrated by the differential effects of pancreatic duct ligation.