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A new method for extracting DNA from the grape berry surface, beginning in the vineyard
Megan E Hall1,2, Lance Cadle-Davidson3, Zhiwei Fang2
1Section of Plant Pathology and Plant-Microbe Biology, School of Integrative Plant Science, Cornell University, NYS Agricultural Experiment Station, Geneva, NY, 14456, United States.
Heliyon
|November 1, 2019
Summary
This study presents a new, cost-effective DNA extraction method for grape surface microbes. The field-to-lab protocol minimizes manipulation, enabling more accurate microbiome studies.
Area of Science:
- Microbiology
- Plant Science
- Genomics
Background:
- Grape surface microbiomes are difficult to study due to microbial adhesion to berry skin.
- Existing methods may alter the natural microbiome before DNA extraction.
Purpose of the Study:
- To develop an efficient, cost-effective field-to-lab DNA extraction protocol for grape surface microbes.
- To minimize sample manipulation and preserve the native grape microbiome.
Main Methods:
- A novel field-to-lab DNA extraction procedure was developed.
- The protocol involves agitation during transport to disrupt the berry surface.
- Common, low-toxicity laboratory chemicals were utilized.
Main Results:
- The protocol efficiently extracts microbial DNA from the grape berry surface.
- It is cost-effective and uses readily available materials.
- Minimal manipulation ensures the integrity of the grape microbiome.
Conclusions:
- This method simplifies and improves the study of grape surface microbiomes.
- It enables more accurate analysis of microbial communities on grape berries.
- The protocol is suitable for researchers in vineyard settings.
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