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Updated: Jan 4, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Single-cell assays using integrated continuous-flow microfluidics.

Ee Xien Ng¹, Myat Noe Hsu¹, Guoyun Sun²

¹Biosystems and Micromechanics, Singapore-MIT Alliance for Research and Technology, Singapore.

Methods in Enzymology

|November 1, 2019

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Continuous-flow microfluidics enables high-throughput single-cell analysis, revealing links between enzyme activity and genetic profiles. This technology offers powerful tools for quantitative biology, synthetic biology, and diagnostics.

Area of Science:

Biotechnology
Molecular Biology
Systems Biology

Background:

Single-cell analysis is crucial for understanding cellular heterogeneity.
Microfluidic technologies have advanced rapidly, enabling sophisticated single-cell profiling.
Integrating various single-cell data types presents significant experimental and computational challenges.

Purpose of the Study:

To review recent advances in continuous-flow microfluidic approaches for single-cell analysis.
To focus on the measurement and statistical analysis of single-cell enzyme activity.
To highlight applications in quantitative biology, synthetic biology, and diagnosis.

Main Methods:

Utilizing continuous-flow microfluidic systems for high-throughput single-cell screening and sorting.

Keywords:

Continuous flow microfluidics Drop-screen Integrative microfluidic procedure Single cell enzyme activity assay

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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

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Published on: October 15, 2013

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

Profiling single cells for genetic types, protein expression, and enzyme activities.

Developing integrative approaches for analyzing large-scale single-cell data.

Main Results:

Demonstrated ability to correlate cellular phenotypes (e.g., enzyme activity, secretion) with genetic fingerprints.
Enabled simultaneous measurement of multiple single-cell parameters.
Facilitated the processing and analysis of large single-cell datasets.

Conclusions:

Continuous-flow microfluidics provides transformative capabilities for single-cell analysis.
Integrated approaches are essential for leveraging the full potential of single-cell data.
This technology has significant applications in advancing quantitative biology, synthetic biology, and medical diagnostics.