Microbial electrochemical stimulation of caproate production from ethanol and carbon dioxide

The production of value added chemicals from CO₂ is of critical importance for the practical application of microbial electrosynthesis (MES). Here, a binary electron donor (ED) design (using electrode and ethanol) was introduced to provide an efficient caproate production with the bioconversion of both CO₂ and ethanol. A maximum caproate production rate of 2.41 ± 0.69 g L^-1 d^-1, and a final concentration of 7.66 ± 1.38 g L^-1 was achieved. Caproate production selectivity based on the substrate increased to 91.47 ± 0.58% (Binary EDs) from 32.22 ± 32.58% (open circuit Electrode ED). An observed amount of 23.43 ± 0.69% of carbon within the final binary ED products originated from the CO₂. This work proves for the first time the potential of caproate production from CO₂ utilization and ethanol upgrading using solid electrodes to regulate the chain elongation process.