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Intestinal lipoprotein synthesis in control and hypercholesterolemic rats
L L Swift1, M E Gray, V S LeQuire
1Department of Pathology, Vanderbilt University School of Medicine, Nashville, TN 37232.
Biochimica Et Biophysica Acta
|September 23, 1988
Summary
Dietary cholesterol increases cholesteryl esters in intestinal very-low-density lipoproteins (VLDL) but does not alter their apolipoprotein composition or synthesis patterns in hypercholesterolemic rats.
Area of Science:
- Lipid metabolism
- Atherosclerosis research
- Gastrointestinal physiology
Background:
- Intestinal very-low-density lipoproteins (VLDL) play a role in lipid transport.
- Diet-induced hypercholesterolemia alters VLDL composition.
Purpose of the Study:
- To characterize nascent intestinal intermediate-density lipoproteins (IDL) and VLDL in hypercholesterolemic rats.
- To investigate the impact of dietary cholesterol on intestinal lipoprotein apolipoprotein synthesis.
Main Methods:
- Isolation and characterization of intestinal Golgi IDL and VLDL.
- Isotope incorporation studies to analyze apolipoprotein synthesis.
- Comparison between control and diet-induced hypercholesterolemic rats.
Main Results:
- Hypercholesterolemic rat intestinal VLDL and IDL showed enrichment in cholesteryl esters.
- Apolipoprotein patterns (apo B-48, A-I, A-IV) of IDL were consistent with Golgi VLDL.
- Dietary cholesterol did not significantly alter apolipoprotein composition or isotope incorporation patterns.
Conclusions:
- The primary effect of dietary cholesterol is increased cholesteryl ester content in intestinal Golgi lipoproteins.
- Apolipoprotein synthesis and composition of intestinal VLDL remain largely unaffected by dietary cholesterol.
- Further research is needed to understand the metabolic fate and hepatic effects of these altered lipoproteins.