Brainstem serotonergic, catecholaminergic, and inflammatory adaptations during chronic hypercapnia in goats

Despite the prevalence of CO₂ retention in human disease, little is known about the adaptive neurobiological effects of chronic hypercapnia. We have recently shown 30-d exposure to increased inspired CO₂ (InCO₂) leads to a steady-state ventilation that exceeds the level predicted by the sustained acidosis and the acute CO₂/H⁺ chemoreflex, suggesting plasticity within respiratory control centers. Based on data showing brainstem changes in aminergic and inflammatory signaling during carotid body denervation-induced hypercapnia, we hypothesized chronic hypercapnia per se will lead to similar changes. We found that: 1) increased InCO₂ increased IL-1β in the medullary raphe (MR), ventral respiratory column, and cuneate nucleus after 24 h, but not after 30 d of hypercapnia; 2) the number of serotonergic and total neurons were reduced within the MR and ventrolateral medulla following 30 d of increased InCO₂; 3) markers of tryptophan metabolism were altered following 24 h, but not 30 d of InCO₂; and 4) there were few changes in brainstem amine levels following 24 h or 30 d of increased InCO₂. We conclude that these changes may contribute to initiating or maintaining respiratory neuroplasticity during chronic hypercapnia but alone do not account for ventilatory acclimatization to chronic increased InCO₂.-Burgraff, N. J., Neumueller, S. E., Buchholz, K. J., LeClaire, J., Hodges, M. R., Pan, L., Forster, H. V. Brainstem serotonergic, catecholaminergic, and inflammatory adaptations during chronic hypercapnia in goats.