Sphingomonas paeninsulae sp. nov., isolated from soil sampled at Fildes Peninsula, Antarctica

Strain YZ-8^T, isolated from soil sampled at Fildes Peninsula, Antarctica, was found to be a Gram-stain-negative, yellow-pigmented, oxidase- and catalase-positive, non-motile, non-spore-forming, rod-shaped and aerobic bacterium. Strain YZ-8^T grewoptimally at pH 7.0 and 20 °C. Tolerance to NaCl was up to 0.3 % (w/v) with optimum growth in the absence of NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YZ-8^T belonged to the family Sphingomonas. Strain YZ-8^T showed the highest sequence similarities to Sphingomonas molluscorum KMM 3882^T (94.4 %), Sphingomonas oligophenolica JCM 12082^T (94.4 %), Sphingomonas gotjawalisoli SN6-9^T (94.3 %) and Sphingomonas aquatica W1-2-1^T (94.3 %). The predominant respiratory isoprenoid quinone and polyamine components were identified as ubiquinone Q-10 and sym-homospermidine, respectively. In addition, carotenoid were also found. The polar lipid profile of the strain YZ-8^T was found to contain one phosphatidylethanolamine, an unidentified phospholipid, one diphosphatidylglycerol, one phosphatidylglycerol, two sphingophosphonolipids, one phosphatidylcholine and two unidentified alkali-stable lipids. The G+C content of the genomic DNA was determined to be 58.8 mol%. The main fatty acids were summed feature 8 (comprising C_18 : 1ω7c and/or C_18 : 1ω6c; 35.4 %), summed feature 3 (comprising C_16 : 1ω7c and/or C_16 : 1ω6c; 32.6 %) and C_14 : 0 2-OH (7.7 %). On the basis of the evidence presented in this study, a novel species of the genus Sphingomonas, Sphingomonaspaeninsulae sp. nov., is proposed, with the type strain YZ-8^T (=CCTCC AB 2017137^T=LMG 31027^T).