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Related Concept Videos

Complement System01:27

Complement System

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The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a...
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Complementation Tests00:49

Complementation Tests

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A complementation test is a simple cross to identify whether the two mutations are located on the same gene or different genes. It was first performed by Edward Lewis in the 1940s while working on fruit flies. He developed the test to identify the location and arrangement of different mutations on chromosomes.
Organisms heterozygous for different mutations are crossed pairwise in all combinations. If present on different genes, the mutations can complement each other by providing the missing...
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Editorial: Current challenges in complement diagnostics.

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Related Experiment Video

Updated: Jan 4, 2026

Measuring the 50% Haemolytic Complement CH50 Activity of Serum
08:26

Measuring the 50% Haemolytic Complement CH50 Activity of Serum

Published on: March 29, 2010

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Clinical Complement Analysis-An Overview.

Lillemor Skattum1

  • 1Department of Laboratory Medicine, Section of Microbiology, Immunology and Glycobiology, Lund University, and Clinical Immunology and Transfusion Medicine, Region Skåne, Lund, Sweden.

Transfusion Medicine Reviews
|November 2, 2019
PubMed
Summary
This summary is machine-generated.

Clinical complement analysis is crucial for diagnosing diseases and monitoring therapies. This review details current methods and highlights needs for improved complement diagnostics, including autoantibody assessment and high-throughput screening.

Keywords:
Clinical complement analysisComplement activation/consumptionComplement deficiencyComplement diagnosticsComplement function

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Area of Science:

  • Immunology
  • Clinical Diagnostics

Background:

  • The complement system is integral to various diseases, including inflammatory, autoimmune, and immune deficiency disorders.
  • Complement analysis is increasingly vital for monitoring complement-directed therapies and identifying transplant complications.

Purpose of the Study:

  • To provide a comprehensive overview of current complement analysis methods used in clinical laboratories.
  • To identify areas needing development in complement diagnostics, such as methods for specific analytes and high-throughput screening.

Main Methods:

  • Review of established and emerging complement analysis techniques.
  • Detailed discussion on complement autoantibody assessment methods.
  • Evaluation of complement function, component quantification, and genetic analysis.

Main Results:

  • Current clinical utility encompasses complement function, component and activation fragment quantification, autoantibody identification, and genetic analysis.
  • Shortages exist in commercially available high-throughput methods for complement deficiency screening and activation assessment.
  • Ongoing development aims to address these limitations and improve standardization.

Conclusions:

  • Optimized and timely complement analysis is critical for clinical laboratories.
  • Further development is needed for practical, routine laboratory methods for specific complement analytes and high-throughput screening.
  • Standardization efforts and quality assurance programs are underway to enhance complement diagnostics.