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Integration of Flow Cytometry and Single Cell Sequencing

Dmitry S Andreyev1, Boris L Zybailov2

  • 1X-BIO Institute, University of Tyumen, Tyumen, Russia, 625003; Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR 72205-7199, USA.

Trends in Biotechnology
|November 2, 2019
PubMed

Keywords:
CITE-seqDrop-seqREAP-seqcytometrysingle-cell sequencing

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Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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