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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking
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Integration of Flow Cytometry and Single Cell Sequencing.

Dmitry S Andreyev1, Boris L Zybailov2

  • 1X-BIO Institute, University of Tyumen, Tyumen, Russia, 625003; Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR 72205-7199, USA.

Trends in Biotechnology
|November 2, 2019
PubMed
Summary

Integrating cell analysis with high-throughput sequencing offers a powerful new bioanalytical tool. This approach simultaneously assesses the phenotype, function, and genetic makeup of individual particles in large populations.

Keywords:
CITE-seqDrop-seqREAP-seqcytometrysingle-cell sequencing

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Area of Science:

  • Biotechnology
  • Genomics
  • Cell Biology

Background:

  • Cytometric analysis provides cell phenotype and function data.
  • High-throughput sequencing reveals genetic and transcriptomic information.
  • Current methods analyze these aspects separately.

Purpose of the Study:

  • To describe the integration of cytometric analysis with high-throughput single particle sequencing.
  • To create a comprehensive bioanalytical tool for individual particle assessment.

Main Methods:

  • Adapting existing cytometric technologies.
  • Integrating with well-established high-throughput single particle sequencing platforms.

Main Results:

  • The proposed integration enables simultaneous assessment of phenotype, functionality, genome, and transcriptome.
  • This approach is applicable to cells, mitochondria, and other polynucleotide-containing particles.
  • The method leverages established technologies for feasibility.

Conclusions:

  • The integration of cytometric analysis and single particle sequencing represents a significant advancement in bioanalysis.
  • This unified approach offers a powerful tool for deep characterization of biological particle populations.
  • Further development could unlock new insights in various biological fields.