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Related Experiment Video

Updated: Jan 4, 2026

High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds

High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds

Published on: August 19, 2025

Molecular harvesting with electroporation for tissue profiling.

Alexander Golberg¹, Julia Sheviryov², Oz Solomon³

¹Porter School of Environment and Earth Sciences, Tel Aviv University, Tel Aviv, Israel. agolberg@tauex.tau.ac.il.

Scientific Reports

|November 2, 2019

Summary

Electroporation enables tissue-specific RNA and protein extraction, offering a less invasive alternative to traditional tissue biopsies for molecular profiling. This novel technique advances personalized medicine and cancer diagnosis by harvesting crucial biomolecules.

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Last Updated: Jan 4, 2026

High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds

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Published on: August 19, 2025

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

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Extraction of Histones from Clinical Specimens for Epigenetic Profiling by Mass Spectrometry

Extraction of Histones from Clinical Specimens for Epigenetic Profiling by Mass Spectrometry

Published on: November 21, 2025

Area of Science:

Biotechnology
Molecular Biology
Oncology

Background:

Personalized medicine relies on molecular profiling of tissue samples (DNA, RNA, proteins) for tailored medical decisions.
Current molecular profiling predominantly uses tissue biopsies, which carry risks like injury, bleeding, inflammation, and potential metastasis.
Identifying predictive biomarkers for cancer therapy response and tumor type is crucial.

Purpose of the Study:

To develop and demonstrate a novel technology for harvesting biomolecules from tissues using electroporation.
To evaluate the efficacy of electroporation for extracting RNA and proteins from various tissue types.

Main Methods:

Developed a tissue electroporation technique combining high-voltage short pulses (500 V/cm, 30 µs, 1 Hz) and low-voltage long pulses (50 V/cm, 10 ms, 1 Hz).
Applied the electroporation method to excised kidney and liver samples.
Tested RNA and protein extraction from excised HepG2 tumors in mice.

Main Results:

Electroporation successfully enabled tissue-specific extraction of RNA and proteins.
Demonstrated successful RNA and protein harvesting from kidney, liver, and HepG2 tumor tissues.
Validated the electroporation method for biomolecule extraction from diverse biological samples.

Conclusions:

Electroporation presents a viable, less invasive method for harvesting biomolecules from tissues compared to traditional biopsies.
This technology has the potential to significantly advance molecular profiling for tumors and their microenvironment.
Further in vivo development could lead to novel diagnostic practices in oncology and personalized medicine.