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Updated: Jan 4, 2026

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
Published on: May 21, 2020
Whole genome sequencing data of Oryza sativa L. cv. Takanari
Hiromi Kajiya-Kanegae1, Shiori Yabe2, Hiroe Yoshida3
1Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Science, The University of Tokyo, Japan.
Abstract:
Oryza sativa L. 'Takanari' is one of the most productive indica cultivars [1,2]. Reciprocal chromosome segment substitution lines (CSSLs) derived from a cross between 'Koshihikari' and 'Takanari' are useful tools for the detection and precise mapping of target quantitative trait loci (QTL) in 'Takanari'. Although the available Os-Nipponbare-Reference-IRGSP-1.0 reference genome is available and useful for evaluating genetic diversity among japonica cultivars, it is not always useful for evaluating genetic diversity harbored by indica cultivars such as 'Takanari'. To reveal sequence variants in 'Takanari' and to exploit these variants in rice breeding programs, the whole genome of 'Takanari' was sequenced using a combination of Illumina HiSeq X Ten (20,983,495 reads and %GC 43) and PacBio (2,847,220 high-quality subreads). NGS data obtained have been deposited in the DNA Data Bank of Japan (DDBJ) under accession number DRA007557.
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