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Updated: Jan 4, 2026

Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic
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miR-488 mediates negative regulation of the AKT/NF-κB pathway by targeting Rac1 in LPS-induced inflammation

Junfeng Liu1,2, Shuai Guo1, Kangfeng Jiang1

  • 1Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.

Journal of Cellular Physiology
|November 2, 2019
PubMed

Insights

MicroRNA-488 (miR-488) overexpression reduces inflammation in bovine endometritis by inhibiting the AKT/NF-κB pathway and reactive oxygen species (ROS) production. This study clarifies miR-488

Area of Science:

  • Reproductive biology
  • Molecular biology
  • Immunology

Background:

  • Endometritis is a common cause of infertility.
  • MicroRNAs (miRNAs) regulate inflammatory diseases.
  • The role of miRNAs in lipopolysaccharide (LPS)-induced endometrial inflammation is not fully understood.

Purpose of the Study:

  • To investigate the miRNA-mediated mechanism of LPS-induced endometrial inflammation.
  • To reveal the role of miR-488 in LPS-induced bovine endometritis.

Main Methods:

  • Real-time quantitative polymerase chain reaction
  • Western blot analysis
  • Immunofluorescence
  • Rac family small GTPase 1 (Rac1) interference
  • Dual-luciferase reporter assay

Main Results:

  • miR-488 was overexpressed in LPS-induced bovine endometritis.
  • Overexpression of miR-488 reduced inflammatory cytokines (IL-1β, IL-6, TNF-α).
  • miR-488 inhibited the AKT/NF-κB pathway, Rac1 expression, and reactive oxygen species (ROS) production.

Conclusions:

  • miR-488 negatively regulates LPS-induced endometrial inflammation.
  • miR-488 acts by inhibiting ROS production and the AKT/NF-κB pathway.
  • Provides insights into the therapeutic potential of miR-488 for endometritis.
Keywords:
AKT/NF-κBROSendometritislipopolysaccharidemiR-488

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