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MicroRNA-598 acts as an inhibitor in retinoblastoma through targeting E2F1 and regulating AKT pathway

Fengge Liu1, Qi Zhang2, Yan Liang3

  • 1Department of Ophthalmology, Zoucheng People's Hospital, Zoucheng, Shandong, China.

Journal of Cellular Biochemistry
|November 2, 2019
PubMed

Insights

MicroRNAs (miRNAs) are crucial in cancer. This study shows miR-598 suppresses retinoblastoma (RB) cell growth and spread by targeting E2F1 and the AKT pathway, offering new RB treatment insights.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) play a significant role in cancer development.
  • Retinoblastoma (RB) is a severe pediatric eye cancer with life-threatening potential.

Purpose of the Study:

  • To investigate the role of miR-598 in the progression of retinoblastoma.
  • To explore miR-598's therapeutic potential in RB.

Main Methods:

  • Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for miRNA expression.
  • Western blot for protein analysis.
  • Dual-luciferase reporter assays to confirm target interaction.
  • Cell Counting Kit-8 (CCK-8) and Transwell assays for cell viability and metastasis.
  • Functional assays to assess apoptosis and pathway activity.

Main Results:

  • miR-598 expression was found to be decreased in retinoblastoma tissues.
  • Overexpression of miR-598 inhibited RB cell viability and metastasis.
  • E2F transcription factor 1 (E2F1) was identified as a direct target of miR-598.
  • E2F1 overexpression reversed the inhibitory effects of miR-598 on RB cell progression.
  • miR-598 promoted apoptosis and inactivated the AKT pathway in RB cells.

Conclusions:

  • miR-598 suppresses retinoblastoma cell viability and metastasis by inhibiting E2F1 and inactivating the AKT pathway.
  • miR-598 represents a potential therapeutic target for retinoblastoma treatment.
Keywords:
AKT pathwayE2F1miR-598retinoblastoma

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