Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Augmenting large language models with clinical knowledge graph for personalized perioperative fluid therapy question answering.

PLOS digital health·2026

Same author

PEPRKD-depression: A knowledge database supporting evidence-based personalized exercise prescription recommendations in depression.

Digital health·2026

Same author

Navigating the complexities of dual CNV findings: a case of DEE7 caused by a <i>de novo KCNQ2</i> deletion and a Co-occurring chromosome 13 duplication - a case report and literature review.

Frontiers in pediatrics·2026

Same author

FPGDKG 1.0: An Integrated Facial Phenotype-Gene-Disease Knowledge Graph for Rare Disease Diagnosis and Explanation.

IEEE journal of biomedical and health informatics·2026

Same author

DRPMKB1.0: A Comprehensive Knowledge Base for an AI-Oriented Drug Repositioning Prediction Model.

Journal of chemical information and modeling·2025

Same author

Bound Polyphenols in Rice Bran Insoluble Dietary Fiber Ameliorate Intestinal Barrier Damage via Butyrate-Mediated Regulation of Apoptosis-Autophagy Signaling.

Journal of agricultural and food chemistry·2025

Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026

Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026

Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026

Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026

Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026

Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 4, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Fast surface profilometry utilizing structured illumination microscopy based on the time-domain phase-shift

Lei Liu, Yan Tang, Zhongye Xie

|November 2, 2019

Summary

This summary is machine-generated.

A novel structured illumination microscopy (SIM) method, SIM-TPT, enhances speed and accuracy for surface profilometry. This technique preserves high-frequency details, enabling measurements on both smooth and rough surfaces.

More Related Videos

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

Published on: December 3, 2013

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

Related Experiment Videos

Last Updated: Jan 4, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

Published on: December 3, 2013

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

Area of Science:

Optics and Photonics
Metrology
Surface Science

Background:

Structured illumination microscopy (SIM) is valuable for high-accuracy surface profilometry.
Conventional SIM methods (phase-shift, Hilbert, global Fourier) face limitations in speed or data loss.
Existing techniques struggle with complex surfaces, limiting their application.

Purpose of the Study:

To introduce a faster and more versatile SIM-based surface profilometry technique.
To overcome the speed and surface-type limitations of existing SIM methods.
To enable high-resolution surface measurements on diverse sample topographies.

Main Methods:

Developed a time-domain phase-shift technique (SIM-TPT) combining single fringe projection and phase shift.
Synchronized object scanning with phase-shifted fringe pattern switching.
Employed a local Fourier transform method for point-to-point modulation information extraction.

Main Results:

Achieved significantly higher measurement speed compared to conventional SIM.
Preserved high-frequency surface information, enabling application to rough surfaces.
Successfully measured complex structures, including steep edges and high roughness.

Conclusions:

SIM-TPT offers a substantial advancement in speed and applicability for surface profilometry.
The method's ability to handle complex surfaces broadens its practical use.
This technique provides a more efficient and versatile solution for detailed surface analysis.